Application
Fig1: Western blot analysis of Histone H2A(hydroxyl Y39) on different lysates using anti-Histone H2A(hydroxyl Y39) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.| Product Name | Histone H2A(hydroxyl Y39) [SR4-17] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2A/m antibody H2A1B_HUMAN antibody HIST1H2AE antibody Histone H2A type 1-B/E antibody Histone H2A.2 antibody Histone H2A.m antibody Histone H2A/a antibody Histone H2A/m antibody |
| Product description | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | H2A/m antibody H2A1B_HUMAN antibody HIST1H2AE antibody Histone H2A type 1-B/E antibody Histone H2A.2 antibody Histone H2A.m antibody Histone H2A/a antibody Histone H2A/m antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | NIH/3T3, Hela, human tonsil tissue, human colon cancer tissue, mouse testis tissue, mouse colon tissue. |
Application
Fig1: Western blot analysis of Histone H2A(hydroxyl Y39) on different lysates using anti-Histone H2A(hydroxyl Y39) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(hydroxyl Y39) antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, Hela, human tonsil tissue, human colon cancer tissue, mouse testis tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
