Application
Fig1: Western blot analysis of PP2A alpha + beta on different lysates using anti-PP2A alpha + beta antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: NIH/3T3 Lane 3: 293TApplication
Fig2: ICC staining PP2A alpha + beta in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PP2A alpha + beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PP2A alpha + beta in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining PP2A alpha + beta in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.Application
Fig11: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.Application
Fig12:Western blot analysis of PP2A alpha + beta on hybrid fish (crucian-carp) brain tissue lysate using anti-PP2A alpha + beta antibody at 1/500 dilution.| Product Name | PP2A alpha + beta [SN70-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PP2A A antibody PP2A alpha antibody PP2A B antibody PP2A beta antibody PP2A-alpha antibody PP2AA_HUMAN antibody PP2Ac antibody PP2CB antibody PPP2CA antibody PPP2CB antibody Protein phosphatase 2 catalytic subunit alpha isoform antibody Protein phosphatase 2 catalytic subunit beta isoform antibody Replication protein C antibody RP C antibody RP-C antibody Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody |
| Product description | The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | PP2A A antibody PP2A alpha antibody PP2A B antibody PP2A beta antibody PP2A-alpha antibody PP2AA_HUMAN antibody PP2Ac antibody PP2CB antibody PPP2CA antibody PPP2CB antibody Protein phosphatase 2 catalytic subunit alpha isoform antibody Protein phosphatase 2 catalytic subunit beta isoform antibody Replication protein C antibody RP C antibody RP-C antibody Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Cytoplasm, Nucleus, Chromosome, Cytoskeleton. |
Application
Fig1: Western blot analysis of PP2A alpha + beta on different lysates using anti-PP2A alpha + beta antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: NIH/3T3 Lane 3: 293T
Application
Fig2: ICC staining PP2A alpha + beta in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PP2A alpha + beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PP2A alpha + beta in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining PP2A alpha + beta in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.
Application
Fig11: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.
Application
Fig12:Western blot analysis of PP2A alpha + beta on hybrid fish (crucian-carp) brain tissue lysate using anti-PP2A alpha + beta antibody at 1/500 dilution.| Positive Control | A431, NIH/3T3, 293T, PANC-1, Hela, L6, rat kidney tissue, human tonsil tissue, human pancreas tissue, mouse brain tissue, mouse stomach tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
