Application
Fig1: Western blot analysis of p53(acetyl K370) on HepG2 cell lysates using anti-p53(acetyl K370) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates Lane 3: HepG2 untreated whole cell lysatesApplication
Fig2: ICC staining p53(acetyl K370) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining p53(acetyl K370) in PANC-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining p53(acetyl K370) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | p53(acetyl K370) [SR40-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody |
| Product description | p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers. |
| Immunogen | recombinant protein |
| Modification | acetyl K370 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix. |
Application
Fig1: Western blot analysis of p53(acetyl K370) on HepG2 cell lysates using anti-p53(acetyl K370) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates Lane 3: HepG2 untreated whole cell lysates
Application
Fig2: ICC staining p53(acetyl K370) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining p53(acetyl K370) in PANC-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining p53(acetyl K370) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | A431, PANC-1, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
