Application
Fig1: Western blot analysis of Caspase-3 on different cell lysates using anti-Caspase-3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: 293Application
Fig2: ICC staining Caspase-3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.| Product Name | Caspase-3 [SR03-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A830040C14Rik antibody Apopain antibody CASP-3 antibody CASP3 antibody CASP3_HUMAN antibody Casp3a antibody Caspase 3 antibody Caspase 3, apoptosis-related cysteine peptidase antibody Caspase 3, apoptosis-related cysteine protease antibody Caspase 3, apoptosis-related cysteine protease a antibody Caspase-3 subunit p12 antibody CC3 antibody CPP-32 antibody CPP32 antibody CPP32B antibody Cysteine protease CPP32 antibody EC 3.4.22.56 antibody LICE antibody mldy antibody OTTHUMP00000165052 antibody OTTHUMP00000165053 antibody OTTHUMP00000165054 antibody PARP cleavage protease antibody Procaspase3 antibody Protein Yama antibody SCA 1 antibody SCA-1 antibody SREBP cleavage activity 1 antibody Yama antibody |
| Product description | Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:20-1:50: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | A830040C14Rik antibody
Apopain antibody
CASP-3 antibody
CASP3 antibody
CASP3_HUMAN antibody
Casp3a antibody
Caspase 3 antibody
Caspase 3 apoptosis-related cysteine peptidase antibody Caspase 3 apoptosis-related cysteine protease antibody Caspase 3 apoptosis-related cysteine protease a antibody Caspase-3 subunit p12 antibody CC3 antibody CPP-32 antibody CPP32 antibody CPP32B antibody Cysteine protease CPP32 antibody EC 3.4.22.56 antibody LICE antibody mldy antibody OTTHUMP00000165052 antibody OTTHUMP00000165053 antibody OTTHUMP00000165054 antibody PARP cleavage protease antibody Procaspase3 antibody Protein Yama antibody SCA 1 antibody SCA-1 antibody SREBP cleavage activity 1 antibody Yama antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytoplasm |
Application
Fig1: Western blot analysis of Caspase-3 on different cell lysates using anti-Caspase-3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: 293
Application
Fig2: ICC staining Caspase-3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.| Positive Control | Jurkat, Hela, HepG2, human tonsil tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:20-1:50: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
