Application
Fig1: Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: PC-12 Lane 4: NIH/3T3Application
Fig2:Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution.Positive control:Lane 1:Hybrid fish (crucian-carp) brain tissueLane 2: Hybrid fish (crucian-carp) kidney tissue| Product Name | Actin [JF47-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,IP |
| WB:1:5,000-1:10,000 IP:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat,Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | a actin antibody
ACTA antibody
ACTA1 antibody
Actin alpha skeletal muscle antibody
Actin antibody
actin alpha 1 skeletal muscle 1 antibody actin alpha 1 skeletal muscle antibody Actin alpha skeletal muscle antibody actina antibody actine antibody ACTS_HUMAN antibody aktin antibody Alpha Actin 1 antibody Alpha skeletal muscle Actin antibody alpha skeletal muscle antibody alpha-actin antibody Alpha-actin-1 antibody ASMA antibody CFTD antibody CFTD1 antibody CFTDM antibody MPFD antibody NEM1 antibody NEM2 antibody NEM3 antibody nemaline myopathy type 3 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: PC-12 Lane 4: NIH/3T3
Application
Fig2:Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution.Positive control:Lane 1:Hybrid fish (crucian-carp) brain tissueLane 2: Hybrid fish (crucian-carp) kidney tissue| Positive Control | PC-12, NIH/3T3, Hela, zebrafish. |
|---|---|
| Application Notes | WB:1:5,000-1:10,000 IP:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
