Application
Fig1: Western blot analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: HelaApplication
Fig2: ICC staining HP1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HP1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with HP1 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | HP1 alpha [SR44-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen p25 antibody CBX5 antibody CBX5_HUMAN antibody CG8409 antibody Chromobox 5 antibody Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody Chromobox homolog 5 antibody Chromobox protein homolog 5 antibody Epididymis luminal protein 25 antibody HEL25 antibody Heterochromatin protein 1 alpha antibody Heterochromatin protein 1 antibody Heterochromatin protein 1 homolog alpha antibody HP1 alpha antibody HP1 alpha homolog antibody HP1 antibody HP1A antibody HP1Hs alpha antibody Su(var)205 antibody |
| Product description | The family of Heterochromatin Protein 1 (HP1) ("Chromobox Homolog", CBX) are highly conserved adapter proteins, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.Three different paralogs of HP1 are found in mammals, HP1α, HP1βand HP1γ. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Antigen p25 antibody
CBX5 antibody
CBX5_HUMAN antibody
CG8409 antibody
Chromobox 5 antibody
Chromobox homolog 5 (HP1 alpha homolog Drosophila) antibody Chromobox homolog 5 antibody Chromobox protein homolog 5 antibody Epididymis luminal protein 25 antibody HEL25 antibody Heterochromatin protein 1 alpha antibody Heterochromatin protein 1 antibody Heterochromatin protein 1 homolog alpha antibody HP1 alpha antibody HP1 alpha homolog antibody HP1 antibody HP1A antibody HP1Hs alpha antibody Su(var)205 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Nucleus, Chromosome |
Application
Fig1: Western blot analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela
Application
Fig2: ICC staining HP1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HP1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with HP1 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, Hela, human tonsil tissue, mouse uterus tissue, human breast carcinoma tissue, human uterus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
