Application
Fig1: Western blot analysis of Phospho-eIF-2a(S51) on different lysates using anti-Phospho-eIF-2a(S51) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: PC12Application
Fig2: ICC staining Phospho-eIF-2a(S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Phospho-eIF-2a(S51) [SZ01-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF 2 alpha antibody EIF 2 antibody EIF 2A antibody EIF 2alpha antibody eIF-2-alpha antibody eIF-2A antibody EIF-2alpha antibody EIF2 alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody Eukaryotic translation initiation factor 2 subunit 1 alpha antibody Eukaryotic translation initiation factor 2 subunit 1 antibody Eukaryotic translation initiation factor 2 subunit alpha antibody IF2A_HUMAN antibody |
| Product description | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a. |
| Modification | p-S51 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | EIF 2 alpha antibody EIF 2 antibody EIF 2A antibody EIF 2alpha antibody eIF-2-alpha antibody eIF-2A antibody EIF-2alpha antibody EIF2 alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody Eukaryotic translation initiation factor 2 subunit 1 alpha antibody Eukaryotic translation initiation factor 2 subunit 1 antibody Eukaryotic translation initiation factor 2 subunit alpha antibody IF2A_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Cellular Localization | Cytoplasmic granule |
Application
Fig1: Western blot analysis of Phospho-eIF-2a(S51) on different lysates using anti-Phospho-eIF-2a(S51) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: PC12
Application
Fig2: ICC staining Phospho-eIF-2a(S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, PC12, Hela, human liver tissue, mouse pancreas tissue, mouse brain tissue, mouse placenta tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
