Application Fig1: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Human liver
Application Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig5: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig8: Flow cytometric analysis of Hela cells with Bax antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Product Name | Bax [SZ3-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax (Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activity of Bcl-2. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis regulator BAX antibody BAX antibody Bax-protein antibody BAX_HUMAN antibody BAXA antibody Baxdelta2G9 antibody Baxdelta2G9omega antibody Baxdelta2omega antibody Bcl-2-like protein 4 antibody BCL2 associated X protein antibody BCL2 associated X protein omega antibody BCL2 associated X protein transcript variant delta2 antibody Bcl2-L-4 antibody BCL2L4 antibody membrane isoform alpha antibody |
|---|---|
| Molecular Weight(MW) | 21,15 kDa |
Application Fig1: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Human liver
Application Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig5: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application Fig8: Flow cytometric analysis of Hela cells with Bax antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | MCF-7, Hela, human tonsil tissue, human liver tissue, human liver cancer tissue, human breast carcinoma tissue, mouse skin tissue, mouse prostate tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
