Application Fig1: Western blot analysis of ENO1 on different lysates using anti-ENO1 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain tissue Lane 2: NIH-3T3 Lane 3: Hela Lane 4: HepG2
Application Fig2: Flow cytometric analysis of MCF-7 cells with ENO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application Fig3: Western blot analysis of ENO1 on Zebrafish tissue lysates using anti-ENO1 antibody at 1/1,000 dilution.
| Product Name | ENO1 [JM63-53] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFCICCIF |
| WB:1:500-1:2,000 FC:1:50-1:100 ICC:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 2 phospho D glycerate hydro lyase antibody 2-phospho-D-glycerate hydro-lyase antibody Alpha enolase antibody Alpha enolase like 1 antibody Alpha-enolase antibody C myc promoter binding protein antibody C-myc promoter-binding protein antibody EC 4.2.1.11 antibody eno1 antibody ENO1L1 antibody ENOA_HUMAN antibody Enolase 1 (alpha) antibody Enolase 1 (alpha) like 1 antibody Enolase 1 antibody Enolase alpha antibody MBP 1 antibody MBP-1 antibody MBP1 antibody MBPB1 antibody MPB 1 antibody MPB-1 antibody MPB1 antibody MYC promoter binding protein 1 antibody NNE antibody Non neural enolase antibody Non-neural enolase antibody Phosphopyruvate hydratase antibody Plasminogen binding protein antibody Plasminogen-binding protein antibody PPH antibody Tau crystallin antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
Application Fig1: Western blot analysis of ENO1 on different lysates using anti-ENO1 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain tissue Lane 2: NIH-3T3 Lane 3: Hela Lane 4: HepG2
Application Fig2: Flow cytometric analysis of MCF-7 cells with ENO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application Fig3: Western blot analysis of ENO1 on Zebrafish tissue lysates using anti-ENO1 antibody at 1/1,000 dilution.
| Positive Control | Rat brain tissue lysate, NIH-3T3 cell lysate, Zebrafish tissue lysates, HepG2 cell lysate, Hela cell lysate, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 ICC:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
