Application
Fig1: Western blot analysis of IKB alpha on different lysates using anti-IKB alpha antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: NIH/3T3 Lane 3: HelaApplication
Fig2: ICC staining IKB alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IKB alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining IKB alpha in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.| Product Name | IKB alpha [SZ00-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody |
| Product description | NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of IKB alpha on different lysates using anti-IKB alpha antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: NIH/3T3 Lane 3: Hela
Application
Fig2: ICC staining IKB alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IKB alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IKB alpha in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.| Positive Control | PC-12, MCF-7, Hela, NIH/3T3, SHG-44, human kidney tissue, mouse brain tissue, mouse kidney tissue, mouse stomach tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
