Application
Fig1: Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Mouse brain Lane 3: Human fetal brainApplication
Fig2: ICC staining LOX in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining LOX in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining LOX in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LOX antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-LOX antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig10: Western blot analysis of LOX on Zebrafish tissue lysates using anti-LOX antibody at 1/1,000 dilution.| Product Name | LOX [JU30-23] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | lox antibody LYOX antibody LYOX_HUMAN antibody Lysyl oxidase antibody MGC105112 antibody Protein lysine 6 oxidase antibody Protein-lysine 6-oxidase antibody |
| Product description | The lysyl oxidase family of extracellular proteins includes LOX and four LOX-like enzymes, which are responsible for the deamination of peptidyl lysine residues of collagens and elastin. They also catalyze inter- and intra-crosslinking reactions. Overexpression of LOX may cause severe fibrotic degeneration due to its high resistance to degradative enzymes. Procollagen C-proteinase activity processes LOX from a precursor protein to a mature form. Activation of LOX occurs in normal developing and adult skin, and alterations in LOX expression and activity are associated with skin aging and senescence. LOX is crucial for development of the cardiovascular and respiratory systems. In addition, LOX plays a role in cancer, wound healing, as well as cell motility, chemotaxis and differentiation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | lox antibody LYOX antibody LYOX_HUMAN antibody Lysyl oxidase antibody MGC105112 antibody Protein lysine 6 oxidase antibody Protein-lysine 6-oxidase antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Secreted, extracellular space. |
Application
Fig1: Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Mouse brain Lane 3: Human fetal brain
Application
Fig2: ICC staining LOX in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining LOX in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining LOX in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LOX antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-LOX antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig10: Western blot analysis of LOX on Zebrafish tissue lysates using anti-LOX antibody at 1/1,000 dilution.| Positive Control | Jurkat, mouse brain tissue lysate, human fetal brain tissue, Zebrafish tissue lysates, Hela, HUVEC, SH-SY5Y, rat skeletal tissue, human kidney tissue, human placenta tissue, mouse heart tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
