Application Fig1: Western blot analysis of Smad4 on PC-12 cells lysates using anti-Smad4 antibody at 1/1,000 dilution.
Application Fig2: ICC staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining Smad4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining Smad4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig9: Flow cytometric analysis of SH-SY-5Y cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Product Name | Smad4 [SP05-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | (Small) Mothers Against Decapentaplegic antibody
Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody
Deleted in pancreatic carcinoma locus 4 antibody
Deletion target in pancreatic carcinoma 4 antibody
DPC 4 antibody
DPC4 antibody
hSMAD4 antibody
JIP antibody
MAD homolog 4 antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody
MAD mothers against decapentaplegic homolog 4 antibody
MADH 4 antibody
MADH4 antibody
Med antibody
Medea antibody
Mothers against decapentaplegic homolog 4 antibody
Mothers against decapentaplegic Drosophila homolog of 4 antibody Mothers against DPP homolog 4 antibody MYHRS antibody OTTHUMP00000163548 antibody SMA- and MAD-related protein 4 antibody SMAD 4 antibody SMAD family member 4 antibody SMAD mothers against DPP homolog 4 antibody SMAD4 antibody SMAD4_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
Application Fig1: Western blot analysis of Smad4 on PC-12 cells lysates using anti-Smad4 antibody at 1/1,000 dilution.
Application Fig2: ICC staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining Smad4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining Smad4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Application Fig9: Flow cytometric analysis of SH-SY-5Y cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | SH-SY-5Y, MCF-7, Hela, PC-12, mouse brain tissue, human lung cancer tissue, human breast carcinoma tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
