Application
Fig1: Flow cytometric analysis of Daudi cells with Phospho-NF-kB p65(S529) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Phospho-NF-kB p65(S529) [SP07-00] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody MGC131774 antibody NF kappa B p65delta3 antibody NFKB3 antibody Nuclear Factor NF Kappa B p65 Subunit antibody Nuclear factor NF-kappa-B p65 subunit antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody OTTHUMP00000233473 antibody OTTHUMP00000233474 antibody OTTHUMP00000233475 antibody OTTHUMP00000233476 antibody OTTHUMP00000233900 antibody p65 antibody p65 NF kappaB antibody p65 NFkB antibody relA antibody TF65_HUMAN antibody Transcription factor p65 antibody v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody V rel avian reticuloendotheliosis viral oncogene homolog A antibody v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody |
| Product description | Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser529 of human NF-kB p65. |
| Modification | p-S529 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:500-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
MGC131774 antibody
NF kappa B p65delta3 antibody
NFKB3 antibody
Nuclear Factor NF Kappa B p65 Subunit antibody
Nuclear factor NF-kappa-B p65 subunit antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
OTTHUMP00000233473 antibody
OTTHUMP00000233474 antibody
OTTHUMP00000233475 antibody
OTTHUMP00000233476 antibody
OTTHUMP00000233900 antibody
p65 antibody
p65 NF kappaB antibody
p65 NFkB antibody
relA antibody
TF65_HUMAN antibody
Transcription factor p65 antibody
v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
V rel avian reticuloendotheliosis viral oncogene homolog A antibody
v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
V rel reticuloendotheliosis viral oncogene homolog A nuclear factor of kappa light polypeptide gene enhancer in B cells 3 p65 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
| SwissProt ID | Q04206 |
|---|
Application
Fig1: Flow cytometric analysis of Daudi cells with Phospho-NF-kB p65(S529) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Daudi. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
