Application
Fig1: Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562Application
Fig2: ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ERK1 [SP05-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK 1 antibody ERK antibody ERK-1 antibody ERK1 antibody ERT 2 antibody ERT2 antibody Extracellular Signal Regulated Kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 1 antibody HGNC6877 antibody HS44KDAP antibody HUMKER1A antibody Insulin Stimulated MAP2 Kinase antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 3 antibody MAP Kinase antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 3 antibody MAPK antibody MAPK1 antibody Mapk3 antibody MGC20180 antibody Microtubule Associated Protein 2 Kinase antibody Microtubule-associated protein 2 kinase antibody Mitogen Activated Protein Kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 3 antibody MK03_HUMAN antibody OTTHUMP00000174538 antibody OTTHUMP00000174541 antibody p44 ERK1 antibody p44 MAPK antibody p44-ERK1 antibody p44-MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM 3 antibody PRKM3 antibody Protein Kinase Mitogen Activated 3 antibody |
| Product description | Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p12-p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | ERK 1 antibody ERK antibody ERK-1 antibody ERK1 antibody ERT 2 antibody ERT2 antibody Extracellular Signal Regulated Kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 1 antibody HGNC6877 antibody HS44KDAP antibody HUMKER1A antibody Insulin Stimulated MAP2 Kinase antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 3 antibody MAP Kinase antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 3 antibody MAPK antibody MAPK1 antibody Mapk3 antibody MGC20180 antibody Microtubule Associated Protein 2 Kinase antibody Microtubule-associated protein 2 kinase antibody Mitogen Activated Protein Kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 3 antibody MK03_HUMAN antibody OTTHUMP00000174538 antibody OTTHUMP00000174541 antibody p44 ERK1 antibody p44 MAPK antibody p44-ERK1 antibody p44-MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM 3 antibody PRKM3 antibody Protein Kinase Mitogen Activated 3 antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562
Application
Fig2: ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, MCF-7, Hela, K562, NIH/3T3, human colon cancer tissue, human breast tissue, mouse stomach tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
