Application
Fig1: Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: JurkatApplication
Fig2: Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | RNF20 [SP00-47] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRE1 A antibody BRE1 antibody BRE1 E3 ubiquitin ligase homolog antibody BRE1-A antibody BRE1A antibody BRE1A_HUMAN antibody E3 ubiquitin-protein ligase BRE1A antibody hBRE1 antibody Homolog of S. cerevisiae BRE1 antibody RING finger protein 20 antibody Ring finger protein 20 E3 ubiquitin protein ligase antibody Rnf20 antibody |
| Product description | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF20 (ring finger protein 20), also known as BRE1, BRE1A or hBRE1, is a 975 amino acid nuclear protein that belongs to the BRE1 family. As a component of the RNF20/40 complex, RNF20 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B. RNF20 is required for transcriptional activation of Hox genes and is most likely recruited by p53 to the MDM2 promoter, thereby acting as a transcriptional co-activator. RNF20 contains one zinc finger domain and exists as a homodimer. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:1,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | BRE1 A antibody BRE1 antibody BRE1 E3 ubiquitin ligase homolog antibody BRE1-A antibody BRE1A antibody BRE1A_HUMAN antibody E3 ubiquitin-protein ligase BRE1A antibody hBRE1 antibody Homolog of S. cerevisiae BRE1 antibody RING finger protein 20 antibody Ring finger protein 20 E3 ubiquitin protein ligase antibody Rnf20 antibody |
|---|---|
| Molecular Weight(MW) | 114 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Jurkat
Application
Fig2: Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Jurkat, MCF-7, Hela. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
