Application
Western Blot analysis of recombinant murine TNF-alpha using rabbit TNF alpha antibody.| Product Name | TNF alpha |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC1 antibody APC1 protein antibody Cachectin antibody DIF antibody Differentiation inducing factor antibody Macrophage cytotoxic factor antibody Tnf antibody TNF superfamily, member 2 antibody TNF, macrophage derived antibody TNF, monocyte derived antibody TNF-a antibody TNF-alpha antibody TNFA antibody TNFA_HUMAN antibody TNFSF2 antibody Tumor necrosis factor alpha antibody Tumor necrosis factor antibody Tumor necrosis factor ligand superfamily member 2 antibody Tumor Necrosis Factor, Membrane Form antibody Tumor necrosis factor, soluble form antibody |
| Product description | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells and macrophages. TNF-α plays a key regulatory role in infammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The antibody detects active recombinant murine TNF alpha. The detective sensitivity by Western Blot is 5ng. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC/IF |
| WB:1:500-1:1,000 ELISA:1:10,000-20,000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Alternative Names | APC1 antibody
APC1 protein antibody
Cachectin antibody
DIF antibody
Differentiation inducing factor antibody
Macrophage cytotoxic factor antibody
Tnf antibody
TNF superfamily member 2 antibody TNF macrophage derived antibody TNF monocyte derived antibody TNF-a antibody TNF-alpha antibody TNFA antibody TNFA_HUMAN antibody TNFSF2 antibody Tumor necrosis factor alpha antibody Tumor necrosis factor antibody Tumor necrosis factor ligand superfamily member 2 antibody Tumor Necrosis Factor Membrane Form antibody Tumor necrosis factor soluble form antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Secreted, membrane |
| SwissProt ID | P06804 |
|---|
Application
Western Blot analysis of recombinant murine TNF-alpha using rabbit TNF alpha antibody.| Positive Control | Recombinant murine TNF alpha |
|---|---|
| Application Notes | WB:1:500-1:1,000 ELISA:1:10,000-20,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
