Application
Fig1: Western blot analysis on different cell lysates using anti-NSE rabbit polyclonal antibodies.Application
Fig2: ICC staining NSE in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysate using anti-NSE antibody at 1/500 dilution.| Product Name | NSE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2 phospho D glycerate hydrolyase antibody 2-phospho-D-glycerate hydro-lyase antibody Eno 2 antibody ENO2 antibody ENOG antibody ENOG_HUMAN antibody Enolase 2 (gamma, neuronal) antibody Enolase 2 antibody Enolase 2 gamma neuronal antibody Enolase2 antibody Epididymis secretory protein Li 279 antibody Gamma enolase antibody Gamma-enolase antibody HEL S 279 antibody Neural enolase antibody Neuron specific enolase antibody Neuron specific gamma enolase antibody Neuron-specific enolase antibody neuronal enriched enolase antibody Neurone specific enolase antibody NSE antibody |
| Product description | Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. Detection of NSE with antibodies can be used to identify neuronal cells and cells with neuroendocrine differentiation. NSE is produced by small cell carcinomas which are neuroendocrine in origin. NSE is therefore a useful tumor marker for lung cancer patients. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC |
| WB:1:2000 ICC:1:100 |
|
| Species Reactivity | HumanMouseRatZebra FishFish |
| Concentration | 1mg/ml |
| Alternative Names | 2 phospho D glycerate hydrolyase antibody
2-phospho-D-glycerate hydro-lyase antibody
Eno 2 antibody
ENO2 antibody
ENOG antibody
ENOG_HUMAN antibody
Enolase 2 (gamma neuronal) antibody Enolase 2 antibody Enolase 2 gamma neuronal antibody Enolase2 antibody Epididymis secretory protein Li 279 antibody Gamma enolase antibody Gamma-enolase antibody HEL S 279 antibody Neural enolase antibody Neuron specific enolase antibody Neuron specific gamma enolase antibody Neuron-specific enolase antibody neuronal enriched enolase antibody Neurone specific enolase antibody NSE antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Cytoplasm, cell membrane |
| SwissProt ID | P09104 |
|---|
Application
Fig1: Western blot analysis on different cell lysates using anti-NSE rabbit polyclonal antibodies.
Application
Fig2: ICC staining NSE in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysate using anti-NSE antibody at 1/500 dilution.| Positive Control | Hela, NIH/3T3, F9, human brain, mouse brain, A172, SHG-44, N2A hybrid fish (crucian-carp) brain tissue lysate. |
|---|---|
| Application Notes | WB:1:2000 ICC:1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
