Application Fig1: Western blot analysis of VEGF Receptor 1 on mouse lung lysates using anti-VEGF Receptor 1 antibody at 1/1,000 dilution.
Application Fig2: ICC staining VEGF Receptor 1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining VEGF Receptor 1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining VEGF Receptor 1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
Application Fig7: Flow cytometric analysis of A431 cells with VEGF Receptor 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Product Name | VEGF Receptor 1 [SY09-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | EC 2.7.10.1 antibody FLT 1 antibody FLT antibody Flt-1 antibody FLT1 antibody Fms like tyrosine kinase 1 antibody Fms related tyrosine kinase 1 antibody Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody Fms-like tyrosine kinase 1 antibody FRT antibody Soluble VEGF receptor 1 14 antibody Soluble VEGFR1 variant 2 antibody Soluble VEGFR1 variant 21 antibody Tyrosine protein kinase FRT antibody Tyrosine protein kinase receptor FLT antibody Tyrosine-protein kinase FRT antibody Tyrosine-protein kinase receptor FLT antibody Vascular endothelial growth factor receptor 1 antibody Vascular endothelial growth factor vascular permeability factor receptor antibody Vascular permeability factor receptor 1 antibody Vascular permeability factor receptor antibody VEGFR 1 antibody VEGFR-1 antibody VEGFR1 antibody VGFR1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 151 kDa |
Application Fig1: Western blot analysis of VEGF Receptor 1 on mouse lung lysates using anti-VEGF Receptor 1 antibody at 1/1,000 dilution.
Application Fig2: ICC staining VEGF Receptor 1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining VEGF Receptor 1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining VEGF Receptor 1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
Application Fig7: Flow cytometric analysis of A431 cells with VEGF Receptor 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | N2A, RH-35, SHG-44, HepG2, mouse brain tissue, mouse lung tissue, mouse placenta tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
