Application Fig1: Western blot analysis of FUBP1 on different lysates using anti-FUBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Raji
Application Fig2: ICC staining FUBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining FUBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining FUBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig9: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig10: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig11: Flow cytometric analysis of Jurakt cells with FUBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
| Product Name | FUBP1 [SY11-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Activation of FUSE, the far upstream element, is required for the proper ex-pression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP1 (FUSE-binding protein or far upstream element-binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL-60 cells, FBP1 (FBP), FBP2 and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxyl-terminal activation domains. Expression of FBP1 is detected in undifferentiated cells and is substantially decreased following cellular differentiation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DNA helicase V antibody far upstream element (FUSE) binding protein 1 antibody Far upstream element (FUSE) binding protein 4 antibody Far upstream element binding protein 1 antibody far upstream element binding protein antibody Far upstream element-binding protein 1 antibody FBP antibody FUBP antibody Fubp1 antibody FUBP1_HUMAN antibody Fubp4 antibody FUSE binding protein 1 antibody FUSE-binding protein 1 antibody HDH V antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa |
Application Fig1: Western blot analysis of FUBP1 on different lysates using anti-FUBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Raji
Application Fig2: ICC staining FUBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining FUBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining FUBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig9: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig10: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.
Application Fig11: Flow cytometric analysis of Jurakt cells with FUBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
| Positive Control | Raji, MCF-7, Hela, HepG2, human tonsil tissue, human breast carcinoma tissue, mouse stomach tissue, human pancreas tissue, mouse pancreas tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
