Application
Fig1: Western blot analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brainApplication
Fig2: ICC staining Phospho-Smad5(S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho-Smad5(S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.| Product Name | Phospho-Smad5(S463/465) [SY09-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser463 and 465 of human Smad5. |
| Modification | p-S463/465 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC,IHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | DKFZp781C1895 antibody
DKFZp781O1323 antibody
Dwfc antibody
hSmad5 antibody
JV5 1 antibody
JV5-1 antibody
MAD homolog 5 antibody
MAD mothers against decapentaplegic homolog 5 antibody MADH 5 antibody MADH5 antibody Mothers against decapentaplegic homolog 5 antibody mothers against decapentaplegic drosophila homolog of 5 antibody Mothers against DPP homolog 5 antibody MusMLP antibody SMA and MAD related protein 5 antibody SMAD 5 antibody SMAD family member 5 antibody SMAD mothers against DPP homolog 5 antibody Smad5 antibody SMAD5_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brain
Application
Fig2: ICC staining Phospho-Smad5(S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-Smad5(S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.| Positive Control | SKOV-3, HepG2, Human liver tissue, mouse liver tissue, mouse brain tissue, human tonsil tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
