Application
Fig1: Western blot analysis of Calponin on Hela cell lysates using anti-Calponin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Calponin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Calponin antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Calponin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Calponin antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Calponin antibody. Counter stained with hematoxylin.| Product Name | Calponin [SI67-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated Myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin), are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat,Dog,Pig |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Basic calponin antibody Calponin 1 antibody Calponin 1 basic smooth muscle antibody Calponin H1 antibody Calponin H1 smooth muscle antibody Calponin-1 antibody Calponin1 antibody Calponins basic antibody CNN 1 antibody Cnn1 antibody CNN1_HUMAN antibody Epididymis secretory protein Li 14 antibody HEL S 14 antibody Sm Calp antibody SMCC antibody smooth muscle antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Cytoskeleton |
Application
Fig1: Western blot analysis of Calponin on Hela cell lysates using anti-Calponin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Calponin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Calponin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Calponin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Calponin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Calponin antibody. Counter stained with hematoxylin.| Positive Control | HepG2, human breast tissue, mouse bladder tissue, mouse stomach tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
