Application
Fig1: Western blot analysis of NGF on Hela cell lysates using anti-NGF antibody at 1/1,000 dilution.Application
Fig2: ICC staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NGF antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-NGF antibody. Counter stained with hematoxylin.| Product Name | NGF [SI79-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat,Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Beta nerve growth factor antibody Beta NGF antibody Beta-nerve growth factor antibody Beta-NGF antibody HSAN5 antibody MGC161426 antibody MGC161428 antibody Nerve growth factor (beta polypeptide) antibody Nerve growth factor antibody Nerve growth factor beta antibody Nerve growth factor beta polypeptide antibody Nerve growth factor beta subunit antibody NGF antibody NGF_HUMAN antibody NGFB antibody NID67 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of NGF on Hela cell lysates using anti-NGF antibody at 1/1,000 dilution.
Application
Fig2: ICC staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NGF antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-NGF antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, Hela, HepG2, mouse thymus tissue, mouse liver tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
