Application
Fig1: Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: LovoApplication
Fig2: ICC staining CD8 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD8 alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. Counter stained with hematoxylin.| Product Name | CD8 alpha [SI18-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | alpha polypeptide (p32) antibody CD8 antibody CD8 antigen alpha polypeptide antibody CD8 antigen alpha polypeptide (p32) antibody CD8a antibody CD8A antigen antibody CD8A molecule antibody CD8A_HUMAN antibody Leu2 antibody Leu2 T lymphocyte antigen antibody Ly3 antibody LYT3 antibody MAL antibody OKT8 T cell antigen antibody OTTHUMP00000160760 antibody OTTHUMP00000160764 antibody OTTHUMP00000203528 antibody OTTHUMP00000203721 antibody p32 antibody T cell antigen Leu2 antibody T cell co receptor antibody T-cell surface glycoprotein CD8 alpha chain antibody T-lymphocyte differentiation antigen T8/Leu-2 antibody T8 T cell antigen antibody T8/Leu-2 T-lymphocyte differentiation antigen antibody |
| Product description | The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 (CD8-α and CD8-β) co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8 proteins; T helper cells express CD4 proteins and T cytotoxic cells display CD8 proteins. CD8s are cell surface glycoproteins that exist as two chain complex (αα or αβ) receptors that bind class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 proteins is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | alpha polypeptide (p32) antibody CD8 antibody CD8 antigen alpha polypeptide antibody CD8 antigen alpha polypeptide (p32) antibody CD8a antibody CD8A antigen antibody CD8A molecule antibody CD8A_HUMAN antibody Leu2 antibody Leu2 T lymphocyte antigen antibody Ly3 antibody LYT3 antibody MAL antibody OKT8 T cell antigen antibody OTTHUMP00000160760 antibody OTTHUMP00000160764 antibody OTTHUMP00000203528 antibody OTTHUMP00000203721 antibody p32 antibody T cell antigen Leu2 antibody T cell co receptor antibody T-cell surface glycoprotein CD8 alpha chain antibody T-lymphocyte differentiation antigen T8/Leu-2 antibody T8 T cell antigen antibody T8/Leu-2 T-lymphocyte differentiation antigen antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa |
| Cellular Localization | Cell membrane, Secreted. |
| SwissProt ID | P01732 |
|---|
Application
Fig1: Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Lovo
Application
Fig2: ICC staining CD8 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD8 alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. Counter stained with hematoxylin.| Positive Control | Lovo, CRC, Hela, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
