Application
Fig1: Western blot analysis of CD86 on different lysates using anti-CD86 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: JurkatApplication
Fig2: ICC staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CD86 [SJ20-00] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activation B7-2 antigen 3 antibody Activation B7-2 antigen antibody B-lymphocyte activation antigen B7-2 2 antibody B-lymphocyte activation antigen B7-2 antibody B7 2 antibody B7 antibody B7-2 antibody B7.2 antibody B70 antibody B72 antigen antibody BU63 antibody CD28 antigen ligand 2 2 antibody CD28 antigen ligand 2 antibody Cd28l2 antibody CD28LG2 antibody CD86 antibody CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody CD86 antigen antibody CD86 molecule antibody CD86_HUMAN antibody CLS1 antibody CTLA-4 counter-receptor B7.2 2, 3 antibody CTLA-4 counter-receptor B7.2 antibody Early T-cell costimulatory molecule 1 antibody ETC-1 antibody FUN 1 antibody FUN-1 antibody LAB72 antibody Ly-58 antibody Ly58 antibody MB7 antibody MB7-2 antibody Membrane glycoprotein antibody MGC34413 antibody T-lymphocyte activation antigen CD86 antibody TS/A-2 antibody |
| Product description | T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins B7-1 (CD80) and B7-2 (CD86) expressed on antigen presenting cells bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28, and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRatCynomolgus Monkey |
| Concentration | 1mg/ml |
| Alternative Names | Activation B7-2 antigen 3 antibody
Activation B7-2 antigen antibody
B-lymphocyte activation antigen B7-2 2 antibody
B-lymphocyte activation antigen B7-2 antibody
B7 2 antibody
B7 antibody
B7-2 antibody
B7.2 antibody
B70 antibody
B72 antigen antibody
BU63 antibody
CD28 antigen ligand 2 2 antibody
CD28 antigen ligand 2 antibody
Cd28l2 antibody
CD28LG2 antibody
CD86 antibody
CD86 antigen (CD28 antigen ligand 2 B7-2 antigen) 1 2 antibody CD86 antigen (CD28 antigen ligand 2 B7-2 antigen) antibody CD86 antigen antibody CD86 molecule antibody CD86_HUMAN antibody CLS1 antibody CTLA-4 counter-receptor B7.2 2 3 antibody CTLA-4 counter-receptor B7.2 antibody Early T-cell costimulatory molecule 1 antibody ETC-1 antibody FUN 1 antibody FUN-1 antibody LAB72 antibody Ly-58 antibody Ly58 antibody MB7 antibody MB7-2 antibody Membrane glycoprotein antibody MGC34413 antibody T-lymphocyte activation antigen CD86 antibody TS/A-2 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of CD86 on different lysates using anti-CD86 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Jurkat
Application
Fig2: ICC staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, JAR, Hela, Raji, Jurkat, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
