Application
Fig1: Western blot analysis of SUMO-1 on Hela cell lysates using anti-SUMO-1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining SUMO-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining SUMO-1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining SUMO-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with SUMO-1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | SUMO-1 [SJ20-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAP1 antibody GAP modifying protein 1 antibody GAP-modifying protein 1 antibody GMP 1 antibody GMP1 antibody OFC10 antibody PIC 1 antibody PIC1 antibody SENP2 antibody Sentrin 1 antibody Sentrin antibody Small ubiquitin related modifier 1 antibody Small ubiquitin-like modifier 1 antibody Small ubiquitin-related modifier 1 antibody SMT3 antibody SMT3 homolog 3 antibody SMT3 suppressor of mif two 3 homolog 1 antibody SMT3, yeast, homolog 3 antibody Smt3C antibody SMT3H3 antibody SUMO-1 antibody SUMO1 antibody SUMO1_HUMAN antibody Ubiquitin homology domain protein PIC1 antibody Ubiquitin Like 1 antibody Ubiquitin like protein SMT3C antibody Ubiquitin like protein UBL1 antibody Ubiquitin-homology domain protein PIC1 antibody Ubiquitin-like protein SMT3C antibody Ubiquitin-like protein UBL1 antibody UBL 1 antibody UBL1 antibody |
| Product description | The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer’s disease. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFCChIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DAP1 antibody
GAP modifying protein 1 antibody
GAP-modifying protein 1 antibody
GMP 1 antibody
GMP1 antibody
OFC10 antibody
PIC 1 antibody
PIC1 antibody
SENP2 antibody
Sentrin 1 antibody
Sentrin antibody
Small ubiquitin related modifier 1 antibody
Small ubiquitin-like modifier 1 antibody
Small ubiquitin-related modifier 1 antibody
SMT3 antibody
SMT3 homolog 3 antibody
SMT3 suppressor of mif two 3 homolog 1 antibody
SMT3 yeast homolog 3 antibody Smt3C antibody SMT3H3 antibody SUMO-1 antibody SUMO1 antibody SUMO1_HUMAN antibody Ubiquitin homology domain protein PIC1 antibody Ubiquitin Like 1 antibody Ubiquitin like protein SMT3C antibody Ubiquitin like protein UBL1 antibody Ubiquitin-homology domain protein PIC1 antibody Ubiquitin-like protein SMT3C antibody Ubiquitin-like protein UBL1 antibody UBL 1 antibody UBL1 antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Nucleus membrane, Cytoplasm, Nucleus, Cell membrane. |
Application
Fig1: Western blot analysis of SUMO-1 on Hela cell lysates using anti-SUMO-1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining SUMO-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining SUMO-1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining SUMO-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with SUMO-1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, MCF-7, Hela, mouse thyroid tissue, human tonsil tissue, mouse placenta tissue, human thyroid tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
