Application
Fig1: Western blot analysis of Apaf-1 on HUVEC cell lysates using anti-Apaf-1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Apaf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Apaf-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Apaf-1 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.| Product Name | Apaf-1 [SY22-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFkB in response to bacterial lipopoly-saccharides. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | APAF 1 antibody Apaf-1 antibody APAF_HUMAN antibody Apaf1 antibody Apoptotic peptidase activating factor 1 antibody Apoptotic protease activating factor 1 antibody Apoptotic protease activating factor antibody Apoptotic protease-activating factor 1 antibody CED 4 antibody CED4 antibody KIAA0413 antibody |
|---|---|
| Molecular Weight(MW) | 141 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Apaf-1 on HUVEC cell lysates using anti-Apaf-1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Apaf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Apaf-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Apaf-1 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.| Positive Control | HUVEC, MCF-7, Hela, Ags, mouse skin tissue, human tonsil tissue, mouse heart tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
