Application
Fig1: ICC staining Phospho-JAK2(Y1007+Y1008) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. Counter stained with hematoxylin.WB
Western blot analysis of Phospho-JAK2 (Y1007 + Y1008) on different lysates with Rabbit anti-Phospho-JAK2 (Y1007 + Y1008) antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
(D) HeLa cells were treated with FMN for 12 h. Whole cell extracts of STAT3 upstream kinase were assessed by western blot.| Product Name | Phospho-JAK2(Y1007+Y1008) [SY24-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immuno-logical stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK-Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK-Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 are sites of trans- or autophosphorylation in vivo and in in vitro kinase reactions. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and 1008 of human JAK2. |
| Modification | p-Y1007+Y1008 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICCIHCIPWB |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | JAK 2 antibody JAK-2 antibody JAK2 antibody JAK2_HUMAN antibody Janus Activating Kinase 2 antibody Janus kinase 2 (a protein tyrosine kinase) antibody Janus kinase 2 antibody JTK 10 antibody JTK10 antibody kinase Jak2 antibody OTTHUMP00000043260 antibody THCYT3 antibody Tyrosine protein kinase JAK2 antibody Tyrosine-protein kinase JAK2 antibody |
|---|---|
| Molecular Weight(MW) | 130 kDa |
| Cellular Localization | Endomembrane system, Cytoplasm, Nucleus. |
Application
Fig1: ICC staining Phospho-JAK2(Y1007+Y1008) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. Counter stained with hematoxylin.
WB
Western blot analysis of Phospho-JAK2 (Y1007 + Y1008) on different lysates with Rabbit anti-Phospho-JAK2 (Y1007 + Y1008) antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
(D) HeLa cells were treated with FMN for 12 h. Whole cell extracts of STAT3 upstream kinase were assessed by western blot.| Positive Control | Hela, human tonsil tissue, human spleen tissue, mouse spleen tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
