Application
Fig1: Western blot analysis of NOX4 on PC-12 cell lysates using anti-NOX4 antibody at 1/1,000 dilution.Application
Fig2: ICC staining NOX4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NOX4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NOX4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-NOX4 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-NOX4 antibody. Counter stained with hematoxylin.| Product Name | NADPH oxidase 4/NOX4 [SY0214] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kidney oxidase-1 antibody Kidney superoxide-producing NADPH oxidase antibody KOX 1 antibody KOX antibody Kox-1 antibody NADPH antibody NADPH oxidase 4 antibody Nox4 antibody NOX4_HUMAN antibody Renal NAD(P)H-oxidase antibody RENOX antibody |
| Product description | The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIP |
| WB:1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Kidney oxidase-1 antibody Kidney superoxide-producing NADPH oxidase antibody KOX 1 antibody KOX antibody Kox-1 antibody NADPH antibody NADPH oxidase 4 antibody Nox4 antibody NOX4_HUMAN antibody Renal NAD(P)H-oxidase antibody RENOX antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Cell membrane, Nucleus, Endoplasmic reticulum membrane. |
Application
Fig1: Western blot analysis of NOX4 on PC-12 cell lysates using anti-NOX4 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining NOX4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NOX4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NOX4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-NOX4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-NOX4 antibody. Counter stained with hematoxylin.| Positive Control | A431, A549, Hela, PC-12, rat kidney tissue, rat stomach tissue, human lung tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
