Application
Fig1: Western blot analysis of p-HSF1(S326) on different lysates using anti-p-HSF1(S326) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: BT20 Lane 3: AGSApplication
Fig2: ICC staining p-HSF1(S326) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining p-HSF1(S326) in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining p-HSF1(S326) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p-HSF1(S326) antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with p-HSF1(S326) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary| Product Name | Phospho-HSF1(S326) [SU31-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heat shock factor 1 antibody Heat shock factor protein 1 antibody Heat shock transcription factor 1 antibody HSF 1 antibody hsf1 antibody HSF1_HUMAN antibody HSTF 1 antibody HSTF1 antibody |
| Product description | Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser326 of human HSF1. |
| Modification | p-S326 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Heat shock factor 1 antibody Heat shock factor protein 1 antibody Heat shock transcription factor 1 antibody HSF 1 antibody hsf1 antibody HSF1_HUMAN antibody HSTF 1 antibody HSTF1 antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q00613 |
|---|
Application
Fig1: Western blot analysis of p-HSF1(S326) on different lysates using anti-p-HSF1(S326) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: BT20 Lane 3: AGS
Application
Fig2: ICC staining p-HSF1(S326) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining p-HSF1(S326) in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining p-HSF1(S326) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p-HSF1(S326) antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with p-HSF1(S326) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary| Positive Control | Hela, BT20, MCF-7, AGS, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
