Application
Fig1: Western blot analysis of Caldesmon on NIH/3T3 cell lysates using anti-Caldesmon antibody at 1/1,000 dilution.Application
Fig2: ICC staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Caldesmon [SU03-19] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAD antibody CALD 1 antibody CALD1 antibody CALD1_HUMAN antibody Caldesmon 1 antibody Caldesmon 1 Isoform 1 antibody Caldesmon 1 Isoform 2 antibody Caldesmon 1 Isoform 3 antibody Caldesmon 1 Isoform 4 antibody Caldesmon 1 Isoform 5 antibody Caldesmon antibody Caldesmon1 antibody CDM antibody H CAD antibody HCAD antibody L CAD antibody LCAD antibody MGC21352 antibody NAG22 antibody |
| Product description | Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CAD antibody CALD 1 antibody CALD1 antibody CALD1_HUMAN antibody Caldesmon 1 antibody Caldesmon 1 Isoform 1 antibody Caldesmon 1 Isoform 2 antibody Caldesmon 1 Isoform 3 antibody Caldesmon 1 Isoform 4 antibody Caldesmon 1 Isoform 5 antibody Caldesmon antibody Caldesmon1 antibody CDM antibody H CAD antibody HCAD antibody L CAD antibody LCAD antibody MGC21352 antibody NAG22 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Caldesmon on NIH/3T3 cell lysates using anti-Caldesmon antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NIH/3T3, C2C12, human kidney tissue, mouse kidney tissue, human uterus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
