Application
Fig1: Western blot analysis of LGR5/GPR49 on SH-SY-5Y lysates using anti-LGR5/GPR49 antibody at 1/1,000 dilution.Application
Fig2: Flow cytometric analysis of SH-SY-5Y cells with LGR5/GPR49 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | LGR5/GPR49 [SU32-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FEX antibody G protein coupled receptor 49 antibody G protein coupled receptor 67 antibody g protein-coupled receptor fex antibody G-protein coupled receptor 49 antibody G-protein coupled receptor 67 antibody G-protein coupled receptor HG38 antibody GPR 49 antibody GPR 67 antibody GPR49 antibody GPR67 antibody GRP 49 antibody GRP49 antibody HG 38 antibody HG38 antibody Leucine rich repeat containing G protein coupled receptor 5 antibody Leucine-rich repeat-containing G-protein coupled receptor 5 antibody LGR 5 antibody LGR5 antibody LGR5_HUMAN antibody MGC117008 antibody Orphan G protein coupled receptor HG38 antibody |
| Product description | G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR5 (leucine-rich repeat-containing G-protein coupled receptor 5), also known as GPR49 or GPR67, is a 907 amino acid multi-pass membrane protein that contains 17 leucine-rich repeats and belongs to the G protein-coupled receptor family. Expressed in placenta, skeletal muscle and spinal cord, LGR5 functions as an orphan receptor that is thought to play an important role in embryonic growth control and cellular differentiation. Overexpression of LGR5 is associated with increased tumor susceptibility and malignant transformation, implicating LGR5 as a potent tumor-inducing protein. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FEX antibody G protein coupled receptor 49 antibody G protein coupled receptor 67 antibody g protein-coupled receptor fex antibody G-protein coupled receptor 49 antibody G-protein coupled receptor 67 antibody G-protein coupled receptor HG38 antibody GPR 49 antibody GPR 67 antibody GPR49 antibody GPR67 antibody GRP 49 antibody GRP49 antibody HG 38 antibody HG38 antibody Leucine rich repeat containing G protein coupled receptor 5 antibody Leucine-rich repeat-containing G-protein coupled receptor 5 antibody LGR 5 antibody LGR5 antibody LGR5_HUMAN antibody MGC117008 antibody Orphan G protein coupled receptor HG38 antibody |
|---|---|
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Cell membrane, Golgi apparatus. |
Application
Fig1: Western blot analysis of LGR5/GPR49 on SH-SY-5Y lysates using anti-LGR5/GPR49 antibody at 1/1,000 dilution.
Application
Fig2: Flow cytometric analysis of SH-SY-5Y cells with LGR5/GPR49 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SH-SY-5Y. |
|---|---|
| Application Notes | WB:1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
