Application
Fig1: Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.Application
Fig2: ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | DARPP32 [SU0329] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DARPP 32 antibody DARPP-32 antibody Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody Dopamine and cAMP regulated neuronal phosphoprotein antibody Dopamine and cAMP regulated phosphoprotein antibody Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody Dopamine and cAMP regulated phosphoprotein DARPP32 antibody Dopamine- and cAMP-regulated neuronal phosphoprotein antibody FLJ20940 antibody IPPD antibody Neuronal phosphoprotein DARPP 32 antibody PPP1R1B antibody PPR1B_HUMAN antibody Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody Protein phosphatase 1 regulatory inhibitor subunit 1B antibody Protein phosphatase 1 regulatory subunit 1B antibody |
| Product description | Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DARPP 32 antibody DARPP-32 antibody Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody Dopamine and cAMP regulated neuronal phosphoprotein antibody Dopamine and cAMP regulated phosphoprotein antibody Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody Dopamine and cAMP regulated phosphoprotein DARPP32 antibody Dopamine- and cAMP-regulated neuronal phosphoprotein antibody FLJ20940 antibody IPPD antibody Neuronal phosphoprotein DARPP 32 antibody PPP1R1B antibody PPR1B_HUMAN antibody Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody Protein phosphatase 1 regulatory inhibitor subunit 1B antibody Protein phosphatase 1 regulatory subunit 1B antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SH-SY-5Y, MCF-7, mouse brain tissue, rat brain tissue, human gastric carcinoma tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
