Application
Fig1: Western blot analysis of Phospho-Nrf2(S40) on different lysates using anti-Phospho-Nrf2(S40) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: RajiApplication
Fig2: ICC staining Phospho-Nrf2(S40) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho-Nrf2(S40) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Phospho-Nrf2(S40) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of K562 cells with Phospho-Nrf2(S40) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Phospho-Nrf2(S40) [SU0334] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | erythroid derived 2 antibody HEBP1 antibody like 2 antibody NF E2 related factor 2 antibody NF-E2-related factor 2 antibody NF2L2_HUMAN antibody NFE2 related factor 2 antibody NFE2-related factor 2 antibody Nfe2l2 antibody Nrf 2 antibody NRF2 antibody Nuclear factor (erythroid derived 2) like 2 antibody Nuclear factor antibody nuclear factor erythroid 2 like 2 antibody Nuclear factor erythroid 2 related factor 2 antibody Nuclear factor erythroid 2-related factor 2 antibody Nuclear factor erythroid derived 2 like 2 antibody |
| Product description | The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK. It regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (Mares). A family of NF-E2- related proteins, which are collectively known as the Cap ‘n’ collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind Mare sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B and Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from its repressor protein, Keap1. The gene encoding human Nrf3 maps to chromosome 7p15.2. Nrf3 is highly expressed in placenta, B cells and monocytes. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser40 of human Nrf2. |
| Modification | p-S40 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | erythroid derived 2 antibody HEBP1 antibody like 2 antibody NF E2 related factor 2 antibody NF-E2-related factor 2 antibody NF2L2_HUMAN antibody NFE2 related factor 2 antibody NFE2-related factor 2 antibody Nfe2l2 antibody Nrf 2 antibody NRF2 antibody Nuclear factor (erythroid derived 2) like 2 antibody Nuclear factor antibody nuclear factor erythroid 2 like 2 antibody Nuclear factor erythroid 2 related factor 2 antibody Nuclear factor erythroid 2-related factor 2 antibody Nuclear factor erythroid derived 2 like 2 antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q16236 |
|---|
Application
Fig1: Western blot analysis of Phospho-Nrf2(S40) on different lysates using anti-Phospho-Nrf2(S40) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Raji
Application
Fig2: ICC staining Phospho-Nrf2(S40) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-Nrf2(S40) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Phospho-Nrf2(S40) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of K562 cells with Phospho-Nrf2(S40) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Raji, A549, Hela, HepG2, human tonsil tissue, human kidney tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
