Application
Fig1: ICC staining VEGF Receptor 2 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining VEGF Receptor 2 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining VEGF Receptor 2 in PMVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | VEGF Receptor 2 [SU03-42] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1) (1-3), Flk-1 (also designated VEGF-R2) (4-6) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs) (3,5). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC,IHC,IP |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | CD309 antibody
CD309 antigen antibody
EC 2.7.10.1 antibody
Fetal liver kinase 1 antibody
FLK-1 antibody
FLK1 antibody
FLK1 mouse homolog of antibody Kdr antibody Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody Kinase insert domain receptor antibody KRD1 antibody Ly73 antibody Protein tyrosine kinase receptor FLK1 antibody Protein-tyrosine kinase receptor flk-1 antibody soluble VEGFR2 antibody Tyrosine kinase growth factor receptor antibody Vascular endothelial growth factor receptor 2 antibody VEGFR 2 antibody VEGFR antibody VEGFR-2 antibody VEGFR2 antibody VGFR2_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 152 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell junction, Endoplasmic reticulum, Cell membrane, Early endosome, Secreted. |
| SwissProt ID | P35968 |
|---|
Application
Fig1: ICC staining VEGF Receptor 2 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining VEGF Receptor 2 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining VEGF Receptor 2 in PMVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | A431, HUVEC, PMVEC. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
