Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-B Raf antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Hela cells with B Raf antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | B Raf [SU34-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ95109 antibody 94 kDa B raf protein antibody B raf 1 antibody B Raf proto oncogene serine threonine protein kinase antibody B Raf proto oncogene, serine/threonine kinase antibody B RAF1 antibody B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody BRAF 1 antibody BRAF antibody BRAF_HUMAN antibody BRAF1 antibody cRmil antibody MGC126806 antibody MGC138284 antibody Murine sarcoma viral (v-raf) oncogene homolog B1 antibody Murine sarcoma viral v raf oncogene homolog B1 antibody NS7 antibody Oncogen BRAF antibody oncogene BRAF1 antibody p94 antibody Proto-oncogene B-Raf antibody Proto-oncogene c-Rmil antibody RAFB 1 antibody RAFB1 antibody RMIL antibody Serine/threonine-protein kinase B-raf antibody v raf murine sarcoma viral oncogene homolog B antibody v raf murine sarcoma viral oncogene homolog B1 antibody v-Raf murine sarcoma viral oncogene homolog B1 antibody |
| Product description | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ95109 antibody
94 kDa B raf protein antibody
B raf 1 antibody
B Raf proto oncogene serine threonine protein kinase antibody
B Raf proto oncogene serine/threonine kinase antibody B RAF1 antibody B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody BRAF 1 antibody BRAF antibody BRAF_HUMAN antibody BRAF1 antibody cRmil antibody MGC126806 antibody MGC138284 antibody Murine sarcoma viral (v-raf) oncogene homolog B1 antibody Murine sarcoma viral v raf oncogene homolog B1 antibody NS7 antibody Oncogen BRAF antibody oncogene BRAF1 antibody p94 antibody Proto-oncogene B-Raf antibody Proto-oncogene c-Rmil antibody RAFB 1 antibody RAFB1 antibody RMIL antibody Serine/threonine-protein kinase B-raf antibody v raf murine sarcoma viral oncogene homolog B antibody v raf murine sarcoma viral oncogene homolog B1 antibody v-Raf murine sarcoma viral oncogene homolog B1 antibody |
|---|---|
| Molecular Weight(MW) | 85 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Cell membrane. |
Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with B Raf antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Rat testis tissue, rat brain tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
