Application
Fig1: Western blot analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293TApplication
Fig2: ICC staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.| Product Name | Phospho-c-Jun(S63) [SY0297] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA). |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser63 of human c-Jun. |
| Modification | p-S63 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC,IHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Activator protein 1 antibody AP 1 antibody AP1 antibody cJun antibody Enhancer Binding Protein AP1 antibody Jun Activation Domain Binding Protein antibody JUN antibody Jun oncogene antibody JUN protein antibody Jun proto oncogene antibody JUN_HUMAN antibody JUNC antibody Oncogene JUN antibody p39 antibody Proto oncogene c jun antibody Proto oncogene cJun antibody Proto-oncogene c-jun antibody Transcription Factor AP 1 antibody Transcription factor AP-1 antibody Transcription Factor AP1 antibody V jun avian sarcoma virus 17 oncogene homolog antibody V jun sarcoma virus 17 oncogene homolog (avian) antibody V jun sarcoma virus 17 oncogene homolog antibody V-jun avian sarcoma virus 17 oncogene homolog antibody vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T
Application
Fig2: ICC staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, 293T, Hela, MCF-7, A549, human tonsil tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
