Application
Fig1: Western blot analysis of CaMKⅡ on different lysates using anti-CaMKⅡ antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: PC-12 Lane 3: SHG-44Application
Fig2: ICC staining CaMKⅡ in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CaMKⅡ in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CaMKⅡ in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.| Product Name | CaMKⅡ [SU03-57] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcium/calmodulin dependent protein kinase II alpha antibody Calcium/calmodulin dependent protein kinase II beta antibody Calcium/calmodulin dependent protein kinase II delta antibody Calcium/calmodulin dependent protein kinase II gamma antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody CaM kinase II alpha antibody CaM kinase II antibody CaM kinase II beta antibody CaM kinase II delta antibody CaM kinase II gamma antibody CaM kinase II subunit alpha antibody CaMK-II subunit alpha antibody CAMK2 antibody Camk2a antibody CAMK2B antibody CAMK2D antibody CAMK2G antibody CAMKA antibody KCC2A_HUMAN antibody |
| Product description | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Calcium/calmodulin dependent protein kinase II alpha antibody Calcium/calmodulin dependent protein kinase II beta antibody Calcium/calmodulin dependent protein kinase II delta antibody Calcium/calmodulin dependent protein kinase II gamma antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody CaM kinase II alpha antibody CaM kinase II antibody CaM kinase II beta antibody CaM kinase II delta antibody CaM kinase II gamma antibody CaM kinase II subunit alpha antibody CaMK-II subunit alpha antibody CAMK2 antibody Camk2a antibody CAMK2B antibody CAMK2D antibody CAMK2G antibody CAMKA antibody KCC2A_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytoplasm, Sarcoplasmic reticulum membrane, Cell membrane, Cell junction. |
Application
Fig1: Western blot analysis of CaMKⅡ on different lysates using anti-CaMKⅡ antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: PC-12 Lane 3: SHG-44
Application
Fig2: ICC staining CaMKⅡ in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CaMKⅡ in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CaMKⅡ in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.| Positive Control | PC-12, SHG-44, Hela, SH-SY-5Y, mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
