Application
Fig1: Western blot analysis of Cathepsin D on MCF-7 lysates using anti-Cathepsin D antibody at 1/1,000 dilution.Application
Fig2: ICC staining Cathepsin D in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cathepsin D in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.| Product Name | Cathepsin D [SU0360] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CatD antibody CATD_HUMAN antibody Cathepsin D antibody Cathepsin D heavy chain antibody CD antibody Ceroid lipofuscinosis neuronal 10 antibody CLN10 antibody CPSD antibody ctsd antibody Epididymis secretory sperm binding protein Li 130P antibody HEL S 130P antibody Lysosomal aspartyl peptidase antibody Lysosomal aspartyl protease antibody MGC2311 antibody |
| Product description | The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsins may also cleave some protein precursors, thereby releasing regulatory peptides. The promoter region of the cathepsin D gene contains five Sp1 binding sites and four AP-2 binding sites. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CatD antibody CATD_HUMAN antibody Cathepsin D antibody Cathepsin D heavy chain antibody CD antibody Ceroid lipofuscinosis neuronal 10 antibody CLN10 antibody CPSD antibody ctsd antibody Epididymis secretory sperm binding protein Li 130P antibody HEL S 130P antibody Lysosomal aspartyl peptidase antibody Lysosomal aspartyl protease antibody MGC2311 antibody |
|---|---|
| Molecular Weight(MW) | 28/43/46 kDa |
| Cellular Localization | Lysosome, Melanosome, Extracellular space. |
Application
Fig1: Western blot analysis of Cathepsin D on MCF-7 lysates using anti-Cathepsin D antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Cathepsin D in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cathepsin D in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, PANC-1, AGS, human lung tissue, human liver tissue, human breast carcinoma tissue, human gastric cancer tissue,human pancreas tissue, mouse prostate tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:10-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
