Application
Fig1: Western blot analysis of PARP on different lysates using anti-PARP antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Jurkat Lane 3: HelaApplication
Fig2: ICC staining PARP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARP antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | PARP [SU03-68] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP ribosyltransferase 1 antibody NAD(+) ADP-ribosyltransferase 1 antibody pADPRT 1 antibody pADPRT1 antibody PARP 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly (ADP ribose) polymerase 1 antibody poly (ADP ribose) polymerase family, member 1 antibody Poly [ADP-ribose] polymerase 1 antibody Poly(ADP ribose) polymerase antibody poly(ADP ribose) synthetase antibody poly(ADP ribosyl)transferase antibody Poly[ADP ribose] synthetase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody |
| Product description | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP ribosyltransferase 1 antibody NAD(+) ADP-ribosyltransferase 1 antibody pADPRT 1 antibody pADPRT1 antibody PARP 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly (ADP ribose) polymerase 1 antibody poly (ADP ribose) polymerase family member 1 antibody Poly [ADP-ribose] polymerase 1 antibody Poly(ADP ribose) polymerase antibody poly(ADP ribose) synthetase antibody poly(ADP ribosyl)transferase antibody Poly[ADP ribose] synthetase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody |
|---|---|
| Molecular Weight(MW) | 113/89 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of PARP on different lysates using anti-PARP antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Jurkat Lane 3: Hela
Application
Fig2: ICC staining PARP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARP antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, Jurkat, Hela, human spleen tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
