Application
Fig1: Western blot analysis of Calreticulin on different lysates using anti-Calreticulin antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: HL-60Application
Fig2: ICC staining Calreticulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Calreticulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Calreticulin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining Calreticulin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with Calreticulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Calreticulin [SU37-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Autoantigen RO antibody CALR antibody CALR protein antibody CALR_HUMAN antibody Calregulin antibody Calreticulin antibody cC1qR antibody CRP55 antibody CRT antibody CRTC antibody Endoplasmic reticulum resident protein 60 antibody Epididymis secretory sperm binding protein Li 99n antibody ERp60 antibody FLJ26680 antibody grp60 antibody HACBP antibody HEL S 99n antibody RO antibody Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody Sicca syndrome antigen A antibody SSA antibody |
| Product description | Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Autoantigen RO antibody
CALR antibody
CALR protein antibody
CALR_HUMAN antibody
Calregulin antibody
Calreticulin antibody
cC1qR antibody
CRP55 antibody
CRT antibody
CRTC antibody
Endoplasmic reticulum resident protein 60 antibody
Epididymis secretory sperm binding protein Li 99n antibody
ERp60 antibody
FLJ26680 antibody
grp60 antibody
HACBP antibody
HEL S 99n antibody
RO antibody
Sicca syndrome antigen A (autoantigen Ro calreticulin) antibody Sicca syndrome antigen A antibody SSA antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Endoplasmic reticulum lumen, Cytoplasm, Secreted, Cell surface, Sarcoplasmic reticulum lumen. |
Application
Fig1: Western blot analysis of Calreticulin on different lysates using anti-Calreticulin antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: HL-60
Application
Fig2: ICC staining Calreticulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Calreticulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Calreticulin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining Calreticulin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with Calreticulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | SH-SY-5Y, MCF-7, Hela, HL-60, NIH/3T3, L6, human kidney tissue, human liver tissue, mouse brain tissue, human liver cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
