Application
Fig1: ICC staining p16INK4A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining p16INK4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining p16INK4A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | p16INK4A [SU0702] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCM2 antibody CDK4 inhibitor p16 INK4 antibody CDK4I antibody CDKN2 antibody CDKN2A antibody Cell cycle negative regulator beta antibody CMM2 antibody Cyclin dependent kinase 4 inhibitor A antibody Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody Cyclin Dependent Kinase Inhibitor 2A antibody Cyclin dependent kinase inhibitor 2A isoform 4 antibody Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody Cyclin dependent kinase inhibitor p16 antibody INK4 antibody INK4A antibody MLM antibody MTS1 antibody Multiple tumor suppressor 1 antibody p14 antibody p16 antibody P16INK4 antibody p16INK4a antibody p19 antibody p19Arf antibody TP16 antibody |
| Product description | The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates, promote orderly progression through the cell cycle. The protein p16INK4A, identified as a negative regulator of the cell cycle, has been shown to bind to and inhibit the activity of the Cdk4/cyclin D complex. p19 ARF, which is unrelated to p16, arises from transcription of an alternative reading frame of the p16 gene. Like p16, p19 ARF has been shown to induce cell cycle arrest. Mice lacking p19 ARF but expressing functional p16 have been shown to develop tumors early in life. Further studies have indicated that p19 ARF may be disrupted in a large percentage of human T cell acute lymphoblastic leukemias. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:500- 1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CCM2 antibody CDK4 inhibitor p16 INK4 antibody CDK4I antibody CDKN2 antibody CDKN2A antibody Cell cycle negative regulator beta antibody CMM2 antibody Cyclin dependent kinase 4 inhibitor A antibody Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody Cyclin Dependent Kinase Inhibitor 2A antibody Cyclin dependent kinase inhibitor 2A isoform 4 antibody Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody Cyclin dependent kinase inhibitor p16 antibody INK4 antibody INK4A antibody MLM antibody MTS1 antibody Multiple tumor suppressor 1 antibody p14 antibody p16 antibody P16INK4 antibody p16INK4a antibody p19 antibody p19Arf antibody TP16 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P42771 |
|---|
Application
Fig1: ICC staining p16INK4A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining p16INK4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining p16INK4A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | HepG2, PC-3M, Hela, human colon cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500- 1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
