Application
Fig1: ICC staining Histone H3(acetyl K56) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Histone H3(acetyl K56) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Histone H3(acetyl K56) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H3(acetyl K56) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H3(acetyl K56) antibody. Counter stained with hematoxylin.| Product Name | Histone H3(acetyl K56) [SU30-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H3.3A antibody HIST1 cluster, H3E antibody H3 histone family, member A antibody H3.1 antibody H3/l antibody H3F3 antibody H3FF antibody H3FJ antibody H3FL antibody Histone gene cluster 1, H3 histone family, member E antibody histone H3.1t antibody Histone H3/o antibody FLJ92264 antibody H 3 antibody H3 antibody H3 histone family, member B antibody H3 histone family, member C antibody H3 histone family, member D antibody H3 histone family, member F antibody H3 histone family, member H antibody H3 histone family, member I antibody H3 histone family, member J antibody H3 histone family, member K antibody H3 histone family, member L antibody H3 histone family, member T antibody H3 histone, family 3A antibody H3/A antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FH antibody H3FI antibody H3FK antibody HIST1 cluster, H3A antibody HIST1 cluster, H3B antibody HIST1 cluster, H3C antibody HIST1 cluster, H3D antibody HIST1 cluster, H3F antibody HIST1 cluster, H3G antibody HIST1 cluster, H3H antibody HIST1 cluster, H3I antibody HIST1 cluster, H3J antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1, H3a antibody Histone 1, H3b antibody Histone 1, H3c antibody Histone 1, H3d antibody Histone 1, H3e antibody Histone 1, H3f antibody Histone 1, H3g antibody Histone 1, H3h antibody Histone 1, H3i antibody Histone 3, H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1, H3a antibody Histone cluster 1, H3b antibody Histone cluster 1, H3c antibody Histone cluster 1, H3d antibody Histone cluster 1, H3e antibody Histone cluster 1, H3f antibody Histone cluster 1, H3g antibody Histone cluster 1, H3i antibody Histone cluster 1, H3j antibody Histone gene cluster 1, H3 histone family, member A antibody Histone gene cluster 1, H3 histone family, member B antibody Histone gene cluster 1, H3 histone family, member C antibody Histone gene cluster 1, H3 histone family, member D antibody Histone gene cluster 1, H3 histone family, member F antibody Histone gene cluster 1, H3 histone family, member G antibody Histone gene cluster 1, H3 histone family, member H antibody Histone gene cluster 1, H3 histone family, member I antibody Histone gene cluster 1, H3 histone family, member J antibody Histone gene cluster 1, H3A antibody Histone gene cluster 1, H3B antibody Histone gene cluster 1, H3C antibody Histone gene cluster 1, H3D antibody Histone gene cluster 1, H3E antibody Histone gene cluster 1, H3F antibody Histone gene cluster 1, H3G antibody Histone gene cluster 1, H3H antibody Histone gene cluster 1, H3I antibody Histone gene cluster 1, H3J antibody Histone H 3 antibody Histone H3.1 antibody Histone H3.2 antibody Histone H3.3 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody |
| Product description | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Immunogen | recombinant protein |
| Modification | acetyl K56 |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | H3.3A antibody
HIST1 cluster H3E antibody H3 histone family member A antibody H3.1 antibody H3/l antibody H3F3 antibody H3FF antibody H3FJ antibody H3FL antibody Histone gene cluster 1 H3 histone family member E antibody histone H3.1t antibody Histone H3/o antibody FLJ92264 antibody H 3 antibody H3 antibody H3 histone family member B antibody H3 histone family member C antibody H3 histone family member D antibody H3 histone family member F antibody H3 histone family member H antibody H3 histone family member I antibody H3 histone family member J antibody H3 histone family member K antibody H3 histone family member L antibody H3 histone family member T antibody H3 histone family 3A antibody H3/A antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FH antibody H3FI antibody H3FK antibody HIST1 cluster H3A antibody HIST1 cluster H3B antibody HIST1 cluster H3C antibody HIST1 cluster H3D antibody HIST1 cluster H3F antibody HIST1 cluster H3G antibody HIST1 cluster H3H antibody HIST1 cluster H3I antibody HIST1 cluster H3J antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1 H3a antibody Histone 1 H3b antibody Histone 1 H3c antibody Histone 1 H3d antibody Histone 1 H3e antibody Histone 1 H3f antibody Histone 1 H3g antibody Histone 1 H3h antibody Histone 1 H3i antibody Histone 3 H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1 H3a antibody Histone cluster 1 H3b antibody Histone cluster 1 H3c antibody Histone cluster 1 H3d antibody Histone cluster 1 H3e antibody Histone cluster 1 H3f antibody Histone cluster 1 H3g antibody Histone cluster 1 H3i antibody Histone cluster 1 H3j antibody Histone gene cluster 1 H3 histone family member A antibody Histone gene cluster 1 H3 histone family member B antibody Histone gene cluster 1 H3 histone family member C antibody Histone gene cluster 1 H3 histone family member D antibody Histone gene cluster 1 H3 histone family member F antibody Histone gene cluster 1 H3 histone family member G antibody Histone gene cluster 1 H3 histone family member H antibody Histone gene cluster 1 H3 histone family member I antibody Histone gene cluster 1 H3 histone family member J antibody Histone gene cluster 1 H3A antibody Histone gene cluster 1 H3B antibody Histone gene cluster 1 H3C antibody Histone gene cluster 1 H3D antibody Histone gene cluster 1 H3E antibody Histone gene cluster 1 H3F antibody Histone gene cluster 1 H3G antibody Histone gene cluster 1 H3H antibody Histone gene cluster 1 H3I antibody Histone gene cluster 1 H3J antibody Histone H 3 antibody Histone H3.1 antibody Histone H3.2 antibody Histone H3.3 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody |
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| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: ICC staining Histone H3(acetyl K56) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Histone H3(acetyl K56) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Histone H3(acetyl K56) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H3(acetyl K56) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H3(acetyl K56) antibody. Counter stained with hematoxylin.| Positive Control | A549, Hela, HepG2, human kidney tissue, mouse kidney tissue. |
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| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
