Application
Fig1: ICC staining MCSF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining MCSF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MCSF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCSF antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-MCSF antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MCSF antibody. Counter stained with hematoxylin.| Product Name | MCSF [SU0413] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Colony stimulating factor 1 (macrophage) antibody Colony stimulating factor 1 antibody Colony stimulating factor macrophage specific antibody CSF 1 antibody CSF-1 antibody CSF1 antibody CSF1_HUMAN antibody Csfm antibody Lanimostim antibody M CSF antibody M-CSF antibody Macrophage Colony Stimulating Factor 1 antibody Macrophage colony stimulating factor antibody MCSF antibody MGC31930 antibody Processed macrophage colony-stimulating factor 1 antibody |
| Product description | The macrophage colony-stimulating factor (M-CSF), also designated CSF-1, was originally discovered in serum, urine and other biological fluids as a factor that can stimulate the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. M-CSF is a homodimeric cytokine that is produced by fibroblasts, epithelial cells, bone marrow stromal cells, osteoblasts, keratinocytes, macrophages, T cells and B cells. M-CSF is a glycoprotein required for the proliferation and differentiation of mononuclear phagocytes, including osteoclasts. M-CSF has also been identified as an important mediator of the inflammatory response and can regulate the release of proinflammatory cytokines from macrophages. M-CSF exerts its pleiotropic effects by binding to a single type of high affinity cell surface receptor that is encoded by the c-Fms proto-oncogene. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCICCIP |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Colony stimulating factor 1 (macrophage) antibody Colony stimulating factor 1 antibody Colony stimulating factor macrophage specific antibody CSF 1 antibody CSF-1 antibody CSF1 antibody CSF1_HUMAN antibody Csfm antibody Lanimostim antibody M CSF antibody M-CSF antibody Macrophage Colony Stimulating Factor 1 antibody Macrophage colony stimulating factor antibody MCSF antibody MGC31930 antibody Processed macrophage colony-stimulating factor 1 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane, Secreted. |
| SwissProt ID | P09603 |
|---|
Application
Fig1: ICC staining MCSF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining MCSF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MCSF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCSF antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-MCSF antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MCSF antibody. Counter stained with hematoxylin.| Positive Control | A431, HepG2, Hela, human tonsil tissue, human lung tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
