Application
Fig1: ICC staining Androgen receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Androgen receptor in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Androgen receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of MCF-7 cells with Androgen receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Androgen receptor [ST0453] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIS antibody ANDR_HUMAN antibody Androgen nuclear receptor variant 2 antibody Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody Androgen receptor antibody androgen receptor splice variant 4b antibody AR antibody AR8 antibody DHTR antibody Dihydro testosterone receptor antibody Dihydrotestosterone receptor (DHTR) antibody Dihydrotestosterone receptor antibody HUMARA antibody HYSP1 antibody KD antibody Kennedy disease (KD) antibody NR3C4 antibody Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody Nuclear receptor subfamily 3 group C member 4 antibody SBMA antibody SMAX1 antibody Spinal and bulbar muscular atrophy (SBMA) antibody Spinal and bulbar muscular atrophy antibody Testicular Feminization (TFM) antibody TFM antibody |
| Product description | Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and male reproductive physiology. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind the two biologically active androgens, testosterone (T) and dihydrotestosterone (DHT), with high and nearly identical affinities; however, the rates of association and dissociation of T are about three times more rapid than those of DHT. This difference has resulted in speculation as to whether these differences in binding kinetics could account for the different physiological effects of T and DHT. A striking feature of AR is its rapid degradation in the absence of ligand. It is now well established that androgen binding results in an at least six-fold increase in androgen stability and that ligand-induced stabilization of AR is highly androgen- specific. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AIS antibody
ANDR_HUMAN antibody
Androgen nuclear receptor variant 2 antibody
Androgen receptor (dihydrotestosterone receptor testicular feminization spinal and bulbar muscular atrophy Kennedy disease) antibody Androgen receptor antibody androgen receptor splice variant 4b antibody AR antibody AR8 antibody DHTR antibody Dihydro testosterone receptor antibody Dihydrotestosterone receptor (DHTR) antibody Dihydrotestosterone receptor antibody HUMARA antibody HYSP1 antibody KD antibody Kennedy disease (KD) antibody NR3C4 antibody Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody Nuclear receptor subfamily 3 group C member 4 antibody SBMA antibody SMAX1 antibody Spinal and bulbar muscular atrophy (SBMA) antibody Spinal and bulbar muscular atrophy antibody Testicular Feminization (TFM) antibody TFM antibody |
|---|---|
| Molecular Weight(MW) | 99 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
| SwissProt ID | P10275 |
|---|
Application
Fig1: ICC staining Androgen receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Androgen receptor in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Androgen receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of MCF-7 cells with Androgen receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | N2A, MCF-7, PC-3M, human breast carcinoma tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
