Application
Fig1: ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Aurora A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with Aurora A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody| Product Name | Aurora A [ST46-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIK antibody ARK-1 antibody ARK1 antibody AURA antibody Aurka antibody Aurora 2 antibody Aurora A antibody Aurora kinase A antibody Aurora-related kinase 1 antibody Aurora/IPL1 like kinase antibody Aurora/IPL1-related kinase 1 antibody AURORA2 antibody Breast tumor-amplified kinase antibody BTAK antibody hARK1 antibody IAK antibody IPL1 related kinase antibody MGC34538 antibody OTTHUMP00000031340 antibody OTTHUMP00000031341 antibody OTTHUMP00000031342 antibody OTTHUMP00000031343 antibody OTTHUMP00000031344 antibody OTTHUMP00000031345 antibody OTTHUMP00000166071 antibody OTTHUMP00000166072 antibody PPP1R47 antibody Protein phosphatase 1, regulatory subunit 47 antibody Serine/threonine kinase 15 antibody Serine/threonine kinase 6 antibody Serine/threonine-protein kinase 15 antibody Serine/threonine-protein kinase 6 antibody Serine/threonine-protein kinase aurora-A antibody STK15 antibody STK6 antibody STK6_HUMAN antibody STK7 antibody |
| Product description | Activation of the oncogenic protein kinase Aurora A regulates meiotic and mitotic cell cycles in eukaryotic cells. Specifically, Aurora A plays a key role in G2/M progression. Activation occurs via autophosphorylation, and while 14 sites are subject to this, only the threonine residue at position 295 is required for activity. Though autophosphorylation mediates activation, a number of other proteins influence activation, including the spindle assembly factor TPX2 and p53 |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | AIK antibody
ARK-1 antibody
ARK1 antibody
AURA antibody
Aurka antibody
Aurora 2 antibody
Aurora A antibody
Aurora kinase A antibody
Aurora-related kinase 1 antibody
Aurora/IPL1 like kinase antibody
Aurora/IPL1-related kinase 1 antibody
AURORA2 antibody
Breast tumor-amplified kinase antibody
BTAK antibody
hARK1 antibody
IAK antibody
IPL1 related kinase antibody
MGC34538 antibody
OTTHUMP00000031340 antibody
OTTHUMP00000031341 antibody
OTTHUMP00000031342 antibody
OTTHUMP00000031343 antibody
OTTHUMP00000031344 antibody
OTTHUMP00000031345 antibody
OTTHUMP00000166071 antibody
OTTHUMP00000166072 antibody
PPP1R47 antibody
Protein phosphatase 1 regulatory subunit 47 antibody Serine/threonine kinase 15 antibody Serine/threonine kinase 6 antibody Serine/threonine-protein kinase 15 antibody Serine/threonine-protein kinase 6 antibody Serine/threonine-protein kinase aurora-A antibody STK15 antibody STK6 antibody STK6_HUMAN antibody STK7 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Centrosome, Nucleus. |
Application
Fig1: ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Aurora A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with Aurora A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody| Positive Control | SKOV-3, NIH/3T3, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
