Application
Fig1: Western blot analysis of MAP1LC3A on different lysates using anti-MAP1LC3A antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: Mouse brain Lane 3: Mouse liver Lane 4: Mouse skeletal muscleApplication
Fig2: ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MAP1LC3A in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MAP1LC3A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with MAP1LC3A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | MAP1LC3A [ST47-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG8E antibody Autophagy-related protein LC3 A antibody Autophagy-related ubiquitin-like modifier LC3 A antibody LC3 antibody LC3A antibody MAP1 light chain 3 like protein 1 antibody MAP1 light chain 3-like protein 1 antibody MAP1A/1B light chain 3 A antibody MAP1A/MAP1B LC3 A antibody MAP1A/MAP1B light chain 3 A antibody MAP1ALC3 antibody MAP1BLC3 antibody Map1lc3a antibody Microtubule associated proteins 1A/1B light chain 3 antibody Microtubule-associated protein 1 light chain 3 alpha antibody Microtubule-associated proteins 1A and 1B, light chain 3 antibody Microtubule-associated proteins 1A/1B light chain 3A antibody MLP3A_HUMAN antibody |
| Product description | Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs). The microtubule-associated proteins can be divided into two groups, structural and dynamic. The structural microtubule-associated proteins, MAP-1A, MAP-1B, MAP-2A, MAP-2B and MAP-2C, stimulate tubulin assembly, enhance micro-tubule stability and influence the spatial distribution of microtubules within cells. Both MAP-1 and, to a greater extent, MAP-2 have been implicated as agents of microtubule depolymerization by suppressing the dynamic instability of the microtubules. The suppression of microtubule dynamic instability by the MAP proteins is thought to be associated with phosphorylation of the MAPs. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ATG8E antibody
Autophagy-related protein LC3 A antibody
Autophagy-related ubiquitin-like modifier LC3 A antibody
LC3 antibody
LC3A antibody
MAP1 light chain 3 like protein 1 antibody
MAP1 light chain 3-like protein 1 antibody
MAP1A/1B light chain 3 A antibody
MAP1A/MAP1B LC3 A antibody
MAP1A/MAP1B light chain 3 A antibody
MAP1ALC3 antibody
MAP1BLC3 antibody
Map1lc3a antibody
Microtubule associated proteins 1A/1B light chain 3 antibody
Microtubule-associated protein 1 light chain 3 alpha antibody
Microtubule-associated proteins 1A and 1B light chain 3 antibody Microtubule-associated proteins 1A/1B light chain 3A antibody MLP3A_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Cytoplasm, Endomembrane system, Cytoplasmic vesicle. |
Application
Fig1: Western blot analysis of MAP1LC3A on different lysates using anti-MAP1LC3A antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: Mouse brain Lane 3: Mouse liver Lane 4: Mouse skeletal muscle
Application
Fig2: ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MAP1LC3A in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MAP1LC3A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with MAP1LC3A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, PC12, Hela, SHG-44, mouse brain tissue, human liver tissue, mouse liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
