Application
Fig1: Western blot analysis of PI 3 Kinase p85 beta on different lysates using anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: MCF-7 Lane 4: U937Application
Fig2: ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with PI 3 Kinase p85 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | PI 3 Kinase p85 beta [ST04-77] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p85 antibody p85 beta antibody P85B antibody P85B_HUMAN antibody Phosphatidylinositol 3 kinase antibody Phosphatidylinositol 3 kinase regulatory beta subunit antibody Phosphatidylinositol 3 kinase regulatory subunit beta antibody Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody Phosphatidylinositol 3-kinase regulatory subunit beta antibody Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody Phosphoinositide 3 kinase regulatory subunit 2 antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody PI3 kinase p85 beta subunit antibody PI3 kinase p85 subunit beta antibody PI3-kinase regulatory subunit beta antibody PI3-kinase subunit p85-beta antibody PI3K antibody PI3K regulatory subunit beta antibody PIK3R 2 antibody PIK3R2 antibody PtdIns 3 kinase p85 beta antibody PtdIns-3-kinase regulatory subunit beta antibody PtdIns-3-kinase regulatory subunit p85-beta antibody |
| Product description | Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110α expression is restricted to white blood cells. It has been shown to bind p85α and p85β, but it apparently does not phosphorylate these subunits. p110α seems to have the capacity to autophosphorylate. p110α does not interact with the p85 subunits. It has been shown to be activated by α and β heterotrimeric G proteins. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | p85 antibody
p85 beta antibody
P85B antibody
P85B_HUMAN antibody
Phosphatidylinositol 3 kinase antibody
Phosphatidylinositol 3 kinase regulatory beta subunit antibody
Phosphatidylinositol 3 kinase regulatory subunit beta antibody
Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody Phosphatidylinositol 3-kinase regulatory subunit beta antibody Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody Phosphoinositide 3 kinase regulatory subunit 2 antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody Phosphoinositide 3 kinase regulatory subunit 2 (p85 beta) antibody PI3 kinase p85 beta subunit antibody PI3 kinase p85 subunit beta antibody PI3-kinase regulatory subunit beta antibody PI3-kinase subunit p85-beta antibody PI3K antibody PI3K regulatory subunit beta antibody PIK3R 2 antibody PIK3R2 antibody PtdIns 3 kinase p85 beta antibody PtdIns-3-kinase regulatory subunit beta antibody PtdIns-3-kinase regulatory subunit p85-beta antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Cytosol, nucleus |
Application
Fig1: Western blot analysis of PI 3 Kinase p85 beta on different lysates using anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: MCF-7 Lane 4: U937
Application
Fig2: ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with PI 3 Kinase p85 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Raji, MCF-7, Hela, U937, SHG-44, rat testis tissue, rat brain tissue, rat colon tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
