Application
Fig1: Western blot analysis of Dnmt3a on different lysates using anti-Dnmt3a antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Human brain Lane 3: Human heartApplication
Fig2: ICC staining Dnmt3a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Dnmt3a in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Dnmt3a in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Dnmt3a [ST04-78] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA (cytosine 5) methyltransferase 3 alpha antibody DNA (cytosine 5) methyltransferase 3A antibody DNA (cytosine-5)-methyltransferase 3A antibody DNA cytosine methyltransferase 3A2 antibody DNA methyltransferase 3 alpha antibody DNA methyltransferase 3a antibody DNA methyltransferase HsaIIIA antibody DNA MTase HsaIIIA antibody DNM3A_HUMAN antibody DNMT 3a antibody DNMT antibody Dnmt3a antibody DNMT3A2 antibody M.HsaIIIA antibody MCMT antibody OTTHUMP00000201149 antibody TBRS antibody |
| Product description | Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | DNA (cytosine 5) methyltransferase 3 alpha antibody DNA (cytosine 5) methyltransferase 3A antibody DNA (cytosine-5)-methyltransferase 3A antibody DNA cytosine methyltransferase 3A2 antibody DNA methyltransferase 3 alpha antibody DNA methyltransferase 3a antibody DNA methyltransferase HsaIIIA antibody DNA MTase HsaIIIA antibody DNM3A_HUMAN antibody DNMT 3a antibody DNMT antibody Dnmt3a antibody DNMT3A2 antibody M.HsaIIIA antibody MCMT antibody OTTHUMP00000201149 antibody TBRS antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Dnmt3a on different lysates using anti-Dnmt3a antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Human brain Lane 3: Human heart
Application
Fig2: ICC staining Dnmt3a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Dnmt3a in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Dnmt3a in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | NIH/3T3, Hela, HepG2, human brain tissue, human heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
