Application
Fig1: Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC12 Lane 3: NIH/3T3Application
Fig2: ICC staining DGCR8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining DGCR8 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | DGCR8 [ST04-79] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DGCRK6 antibody C22orf12 antibody D16H22S788E antibody D16Wis2 antibody DGCR 8 antibody Dgcr8 antibody DGCR8 microprocessor complex subunit antibody DGCR8_HUMAN antibody DGCRK 6 antibody DiGeorge syndrome critical region 8 antibody DiGeorge syndrome critical region gene 8 antibody Gy1 antibody Microprocessor complex subunit DGCR8 antibody pasha antibody |
| Product description | DGS8, also designated DiGeorge syndrome critical region 8 protein, plays a role in the etiology of the velocardiofacial/DiGeorge syndrome (VCFS/DGS). It is a ubiquitously expressed protein encoded by the gene DGCR8, which is deleted in DiGeorge syndrome. DiGeorge syndrome is characterized by structural and functional palate anomalies, conotruncal cardiac malformations, immunodeficiency, hypocalcemia, and typical facial anomalies. In mouse, DGS8 is detected primarily in embryonic brain, vessels, thymus and palate. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DGCRK6 antibody C22orf12 antibody D16H22S788E antibody D16Wis2 antibody DGCR 8 antibody Dgcr8 antibody DGCR8 microprocessor complex subunit antibody DGCR8_HUMAN antibody DGCRK 6 antibody DiGeorge syndrome critical region 8 antibody DiGeorge syndrome critical region gene 8 antibody Gy1 antibody Microprocessor complex subunit DGCR8 antibody pasha antibody |
|---|---|
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC12 Lane 3: NIH/3T3
Application
Fig2: ICC staining DGCR8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DGCR8 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | PC12, NIH/3T3, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
