Application
Fig1: Western blot analysis of PRMT5 on A431 cell lysates using anti-PRMT5 antibody at 1/1,000 dilution.Application
Fig2: ICC staining PRMT5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.| Product Name | PRMT5 [ST51-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 72 kDa ICln binding protein antibody 72 kDa ICln-binding protein antibody ANM5_HUMAN antibody Histone synthetic lethal 7, S. cerevisiae, homolog of antibody Histone-arginine N-methyltransferase PRMT5 antibody HMT1 hnRNP methyltransferase like 5 antibody HOMOLOG OF; SKB1 antibody HRMT1L5 antibody IBP72 antibody Jak-binding protein 1 antibody JBP 1 antibody JBP1 antibody PRMT 5 antibody PRMT5 antibody Protein arginine methyltransferase 5 antibody Protein arginine N methyltransferase 5 antibody Protein arginine N methyltransferase 5 N terminally processed antibody Protein arginine N-methyltransferase 5 antibody S. POMBE antibody S. POMBE HOMOLOG OF; SKB1 antibody SHK1 KINASE BINDING PROTEIN 1 antibody Shk1 kinase binding protein 1 homolog antibody Shk1 kinase-binding protein 1 homolog antibody Shk1 kinase/binding protein 1, S. pombe, homolog of antibody SKB 1 antibody SKB1 antibody SKB1 homolog antibody SKB1: SKB1 homolog (S. pombe) antibody SKB1Hs antibody |
| Product description | The formation of the spliceosome includes the assembly of Sm proteins in an ordered manner onto snRNAs. This process is mediated by the survival of a motor neuron (SMN) protein and is enhanced by modification of specific Arginine residues in the Sm proteins to symmetrical dimethylarginines (sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome, a complex comprised of the type II methyltransferase PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation. The gene encoding human PRMT5 maps to chromosome 14q11. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 72 kDa ICln binding protein antibody
72 kDa ICln-binding protein antibody
ANM5_HUMAN antibody
Histone synthetic lethal 7 S. cerevisiae homolog of antibody Histone-arginine N-methyltransferase PRMT5 antibody HMT1 hnRNP methyltransferase like 5 antibody HOMOLOG OF SKB1 antibody HRMT1L5 antibody IBP72 antibody Jak-binding protein 1 antibody JBP 1 antibody JBP1 antibody PRMT 5 antibody PRMT5 antibody Protein arginine methyltransferase 5 antibody Protein arginine N methyltransferase 5 antibody Protein arginine N methyltransferase 5 N terminally processed antibody Protein arginine N-methyltransferase 5 antibody S. POMBE antibody S. POMBE HOMOLOG OF SKB1 antibody SHK1 KINASE BINDING PROTEIN 1 antibody Shk1 kinase binding protein 1 homolog antibody Shk1 kinase-binding protein 1 homolog antibody Shk1 kinase/binding protein 1 S. pombe homolog of antibody SKB 1 antibody SKB1 antibody SKB1 homolog antibody SKB1: SKB1 homolog (S. pombe) antibody SKB1Hs antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Golgi apparatus. |
Application
Fig1: Western blot analysis of PRMT5 on A431 cell lysates using anti-PRMT5 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining PRMT5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.| Positive Control | A431, HepG2, human breast carcinoma tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
